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STUB1 Monoclonal antibody

STUB1 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E6B9

Cat No : 68407-1-Ig

Print datasheet

Synonyms

Antigen NY CO 7, CHIP, CLL associated antigen KW 8, HSPABP2, NY CO 7, SDCCAG7, STUB1, UBOX1

验证数据展示

  • WB analysis using 68407-1-Ig

    WB analysis using 68407-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68407-1-Ig (STUB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of MCF-7 using 68407-1-Ig

    IF Staining of MCF-7 using 68407-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using STUB1 antibody (68407-1-Ig, Clone: 2E6B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy STUB1 Ready-To-Use IHC Kit. STUB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68407-1-Ig targets STUB1 in WB, IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name STIP1 homology and U-box containing protein 1
Synonyms Antigen NY CO 7, CHIP, CLL associated antigen KW 8, HSPABP2, NY CO 7, SDCCAG7, STUB1, UBOX1
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberNM_005861
Gene Symbol STUB1
Gene ID (NCBI) 10273
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UNE7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4.(PMID:10330192) CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14. (PMID:20151404) STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer patients.(PMID:21220432 ) This antibody is specific to STUB1.

实验方案

Product Specific Protocols
WB protocol for STUB1 antibody 68407-1-IgDownload protocol
IF protocol for STUB1 antibody 68407-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols