验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, HEK-293 cells, MCF-7 cells, mouse liver tissue |
| Positive IHC detected in | human colon cancer tissue, human liver tissue, mouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55430-1-AP targets STUB1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | STIP1 homology and U-box containing protein 1 |
| 别名 | CHIP, EC:2.3.2.27, E3 ubiquitin-protein ligase CHIP, CLL-associated antigen KW-8, Carboxy terminus of Hsp70-interacting protein |
| 计算分子量 | 35 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | NM_005861 |
| 基因名称 | STUB1 |
| Gene ID (NCBI) | 10273 |
| RRID | AB_10949225 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UNE7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4.(PMID:10330192) CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14. (PMID:20151404) STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer patients.(PMID:21220432 ) This antibody is specific to STUB1.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for STUB1 antibody 55430-1-AP | Download protocol |
| WB protocol for STUB1 antibody 55430-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy TIGAR exacerbates obesity by triggering LRRK2-mediated defects in macroautophagy and chaperone-mediated autophagy in adipocytes | ||
Nat Commun The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma. | ||
Nat Commun Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover. | ||
Mol Cancer Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation. | ||
Cell Death Dis ZC3H15 promotes glioblastoma progression through regulating EGFR stability. |
