• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68496-1-Ig (same clone as 68496-1-PBS)

    WB analysis using 68496-1-Ig (same clone as 68496-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68496-1-Ig (STT3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68496-1-PBS in a different storage buffer formulation.

  • WB analysis using 68496-1-Ig (same clone as 68496-1-PBS)

    WB analysis using 68496-1-Ig (same clone as 68496-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68496-1-Ig (STT3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68496-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 68496-1-Ig (same clone as 68496-1-PBS)

    FC experiment of A431 using 68496-1-Ig (same clone as 68496-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.8 ug STT3B Monoclonal antibody (68496-1-Ig, Clone:3B7A8) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68496-1-PBS in a different storage buffer formulation.

STT3B Monoclonal antibody, PBS Only

STT3B Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 68496-1-PBS

产品说明书

CloneNo. 3B7A8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

STT, Source of immunodominant MHC-associated peptides homolog, SIMP, EC:2.4.99.18, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

68496-1-PBS targets STT3B in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30866

Product name: Recombinant human STT3B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 173-225 aa of BC015880

Sequence: APDNRETLDHKPRVTNIFPKQKYLSKKTTKRKRGYIKNKLVFKKGKKIFKKTV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
别名 STT, Source of immunodominant MHC-associated peptides homolog, SIMP, EC:2.4.99.18, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
计算分子量 97 kDa
观测分子量 94 kDa
GenBank蛋白编号BC015880
基因名称 STT3B
Gene ID (NCBI) 201595
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8TCJ2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STT3B is a component of the N-oligosaccharyl transferase (OST) enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3B seems to be involved in complex substrate specificity