验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive FC (Intra) detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68496-1-Ig targets STT3B in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag30866 Product name: Recombinant human STT3B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 173-225 aa of BC015880 Sequence: APDNRETLDHKPRVTNIFPKQKYLSKKTTKRKRGYIKNKLVFKKGKKIFKKTV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) |
| 别名 | 3B7A8, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B, EC:2.4.99.18, SIMP, Source of immunodominant MHC-associated peptides homolog |
| 计算分子量 | 97 kDa |
| 观测分子量 | 94 kDa |
| GenBank蛋白编号 | BC015880 |
| 基因名称 | STT3B |
| Gene ID (NCBI) | 201595 |
| RRID | AB_3085207 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8TCJ2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STT3B is a component of the N-oligosaccharyl transferase (OST) enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3B seems to be involved in complex substrate specificity
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for STT3B antibody 68496-1-Ig | Download protocol |
| WB protocol for STT3B antibody 68496-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
