验证数据展示
经过测试的应用
| Positive FC detected in | Human Bone marrow mesenchymal stromal cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-FcA65585 targets STRO-1 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
Human CD34 positive bone marrow cells 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | STRO-1 |
| 别名 | STRO1 |
| 基因名称 | |
| Gene ID (NCBI) | |
| 偶联类型 | CoraLite® Plus 555 Fluorescent Dye |
| 最大激发/发射波长 | 554 nm / 570 nm |
| 激发激光 | Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
STRO-1, first identified in 1991, is a well-known cell surface marker to identify and isolate mesenchymal stem cells (MSCs), also known as mesenchymal precursor cells (MPCs). STRO-1 antigen is present on fibroblast colony-forming unit (CFU-F) cells in adult human BM and potentially defines a MSC precursor subpopulation. Western blot analysis identifies Stro-1 as a 75kd protein in endothelial cells (PMID: 21903091, 2070060).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 555 STRO-1 antibody CL555-FcA65585 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
