• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of SH-SY5Y using 82925-3-RR (same clone as 82925-3-PBS)

    FC experiment of SH-SY5Y using 82925-3-RR (same clone as 82925-3-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug STMN2 Recombinant antibody (82925-3-RR, Clone:230110G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82925-3-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 82925-3-RR (same clone as 82925-3-PBS)

    IF Staining of SH-SY5Y using 82925-3-RR (same clone as 82925-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (82925-3-RR, Clone: 230110G10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82925-3-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 82925-3-PBS

    FC experiment of SH-SY5Y using 82925-3-PBS

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug STMN2 Recombinant antibody, PBS Only (82925-3-PBS, Clone:230110G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

STMN2 Recombinant antibody, PBS Only

STMN2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82925-3-PBS

产品说明书

CloneNo. 230110G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

230110G10, SCG 10, SCG10, SCGN 10, SCGN10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy STMN2 Ready-To-Use IHC Kit. STMN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82925-3-PBS targets STMN2 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 STMN2 fusion protein Ag0929 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 stathmin-like 2
别名 230110G10, SCG 10, SCG10, SCGN 10, SCGN10
计算分子量 21 kDa
GenBank蛋白编号BC006302
基因名称 STMN2
Gene ID (NCBI) 11075
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ93045
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)

实验方案

Product Specific Protocols
FC protocol for STMN2 antibody 82925-3-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols