验证数据展示
经过测试的应用
| Positive IHC detected in | mouse testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-25821 targets STK25 in IHC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag22964 Product name: Recombinant human STK25 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 323-426 aa of BC015793 Sequence: PTIRPSPHSKLHKGTALHSSQKPAEPVKRQPRSQCLSTLVRPVFGELKEKHEQSGGSVGALEELENAFSLAEESCPGISDKLMVHLVERVQRFSHNRNHLTSTR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serine/threonine kinase 25 (STE20 homolog, yeast) |
| 别名 | DKFZp686J1430, SOK 1, SOK1, Ste20 like kinase, STK25, YSK1 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC015793 |
| 基因名称 | STK25 |
| Gene ID (NCBI) | 10494 |
| RRID | AB_2918942 |
| 偶联类型 | Biotin |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00506 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin STK25 antibody Biotin-25821 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
