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CoraLite® Plus 488-conjugated STK11/LKB1 Monoclonal antibody

STK11/LKB1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8E4

Cat No : CL488-66719

Print datasheet

Synonyms

LKB1, PJS, serine/threonine kinase 11, STK11, STK11/LKB1

验证数据展示

  • IF Staining of HeLa using CL488-66719

    IF Staining of HeLa using CL488-66719

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 STK11/LKB1 antibody (CL488-66719, Clone: 1H8E4 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-66719

    FC experiment of HeLa using CL488-66719

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human STK11/LKB1 (CL488-66719, Clone:1H8E4) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of HeLa using CL488-66719

    FC experiment of HeLa using CL488-66719

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human STK11/LKB1 (CL488-66719, Clone:1H8E4) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of HeLa using CL488-66719

    FC experiment of HeLa using CL488-66719

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human STK11/LKB1 (CL488-66719, Clone:1H8E4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STK11 Ready-To-Use IHC Kit. STK11 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66719 targets STK11/LKB1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, Mouse, Rat
Immunogen STK11/LKB1 fusion protein Ag13436 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serine/threonine kinase 11
Synonyms LKB1, PJS, serine/threonine kinase 11, STK11, STK11/LKB1
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 45-49 kDa
GenBank Accession NumberBC007981
Gene Symbol STK11
Gene ID (NCBI) 6794
RRIDAB_2883374
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15831
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 STK11/LKB1 antibody CL488-66719Download protocol
FC protocol for CL Plus 488 STK11/LKB1 antibody CL488-66719Download protocol
Standard Protocols
Click here to view our Standard Protocols