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STIP1 Monoclonal antibody

STIP1 Monoclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B5H2

Cat No : 68155-1-Ig

Print datasheet

Synonyms

HOP, Hsc70/Hsp90 organizing protein, IEF SSP 3521, P60, STI1, STI1L, STIP1

验证数据展示

  • IHC staining of mouse liver using 68155-1-Ig

    IHC staining of mouse liver using 68155-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 68155-1-Ig

    IF Staining of HeLa using 68155-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using STIP1 antibody (68155-1-Ig, Clone: 1B5H2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis using 68155-1-Ig

    WB analysis using 68155-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68155-1-Ig (STIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IP experiment of HeLa using 68155-1-Ig

    IP experiment of HeLa using 68155-1-Ig

    IP result of anti-STIP1 (IP:68155-1-Ig, 5ug; Detection:68155-1-Ig 1:1000) with HeLa cells lysate 1880 ug.

  • IHC staining of mouse liver using 68155-1-Ig

    IHC staining of mouse liver using 68155-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inHeLa cells, Jurkat cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68155-1-Ig targets STIP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen STIP1 fusion protein Ag7851 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name stress-induced-phosphoprotein 1
Synonyms HOP, Hsc70/Hsp90 organizing protein, IEF SSP 3521, P60, STI1, STI1L, STIP1
Calculated Molecular Weight 63 kDa
Observed Molecular Weight61 kDa
GenBank Accession NumberBC002987
Gene Symbol STIP1
Gene ID (NCBI) 10963
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP31948
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STIP1, also named as Hop and NY-REN-11. Its expression is low in the hypothalamus. STIP1 has some isoforms with MW 62 kDa, 68 kDa and 59 kDa. Acts as a co-chaperone for HSP90AA1.Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90, Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. STIP1 interacts with PACRG, EEF1AKMT3, FLCN, FNIP1 and FNIP2 (PMID:24880080).

实验方案

Product Specific Protocols
WB protocol for STIP1 antibody 68155-1-IgDownload protocol
IHC protocol for STIP1 antibody 68155-1-IgDownload protocol
IF protocol for STIP1 antibody 68155-1-IgDownload protocol
IP protocol for STIP1 antibody 68155-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols