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STIM1 Recombinant antibody

STIM1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B13

Cat No : 81156-1-RR

Print datasheet

Synonyms

D11S4896E, GOK, STIM1, stromal interaction molecule 1

验证数据展示

  • WB analysis using 81156-1-RR

    WB analysis using 81156-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81156-1-RR (STIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, HepG2 cells, A549 cells, LNCaP cells, Jurkat cells, K-562 cells, Raji cells, RAW 263.7 cells, HSC-T6 cells, C6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81156-1-RR targets STIM1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen STIM1 fusion protein Ag2140 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name stromal interaction molecule 1
Synonyms D11S4896E, GOK, STIM1, stromal interaction molecule 1
Calculated Molecular Weight 685 aa, 77 kDa
Observed Molecular Weight77 kDa
GenBank Accession NumberBC021300
Gene Symbol STIM1
Gene ID (NCBI) 6786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13586
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.

实验方案

Product Specific Protocols
WB protocol for STIM1 antibody 81156-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols