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验证数据展示

  • WB analysis using 81156-1-RR (same clone as 81156-1-PBS)

    WB analysis using 81156-1-RR (same clone as 81156-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81156-1-RR (STIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81156-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 81156-1-RR (same clone as 81156-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 81156-1-RR (same clone as 81156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 81156-1-RR (STIM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81156-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 81156-1-RR (same clone as 81156-1-PBS)

    IF Staining of HEK-293 using 81156-1-RR (same clone as 81156-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using STIM1 antibody (81156-1-RR, Clone: 1B13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81156-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81156-1-RR

    Affinity and Kinetic Characterization of 81156-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81156-1-RR against Human STIM1 were performed. The affinity constant is 0.844 nM.

STIM1 Recombinant monoclonal antibody, PBS Only

STIM1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81156-1-PBS

产品说明书

CloneNo. 1B13

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

1B13, GOK, IMD10, STIM 1, stim-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

81156-1-PBS targets STIM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2140

Product name: Recombinant human STIM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-350 aa of BC021300

Sequence: DVCVRLALWLLWGLLLHQGQSLSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 stromal interaction molecule 1
别名 1B13, GOK, IMD10, STIM 1, stim-1
计算分子量 685 aa, 77 kDa
观测分子量77 kDa
GenBank蛋白编号BC021300
基因名称 STIM1
Gene ID (NCBI) 6786
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13586
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.

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