验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells, HL-60 cells, Daudi cells |
| Positive IHC detected in | human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells, A431 cells |
Planning an IHC experiment? We recommend our IHCeasy STIL Ready-To-Use IHC Kit. STIL primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66876-1-Ig targets STIL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag19124 Product name: Recombinant human STIL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 928-1277 aa of BC126223 Sequence: LHQPSDNQKIYQDLLGQVNHLLNSSSKETEQPSTKAVIISHECTRTQNVYHTKKKTHHSRLVDKDCVLNATLKQLRSLGVKIDSPTKVKKNAHNVDHASVLACISPEAVISGLNCMSFANVGMSGLSPNGVDLSMEANAIALKYLNENQLSQLSVTRSNQNNCDPFSLLHINTDRSTVGLSLISPNNMSFATKKYMKRYGLLQSSDNSEDEEEPPDNADSKSEYLLNQNLRSIPEQLGGQKEPSKNDHEIINCSNCESVGTNADTPVLRNITNEVLQTKAKQQLTEKPAFLVKNLKPSPAVNLRTGKAEFTQHPEKENEGDITIFPESLQPSETLKQMNSMNSVGTFLDV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SCL/TAL1 interrupting locus |
| 别名 | 1E12C1, SCL-interrupting locus protein, SIL, TAL-1-interrupting locus protein |
| 计算分子量 | 1287 aa, 143 kDa |
| 观测分子量 | 150-155 kDa |
| GenBank蛋白编号 | BC126223 |
| 基因名称 | STIL |
| Gene ID (NCBI) | 6491 |
| RRID | AB_2882210 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q15468 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for STIL antibody 66876-1-Ig | Download protocol |
| IHC protocol for STIL antibody 66876-1-Ig | Download protocol |
| WB protocol for STIL antibody 66876-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Int ncRNAs-mediated overexpression of STIL predict unfavorable prognosis and correlated with the efficacy of immunotherapy of hepatocellular carcinoma | ||
Cancer Cell Int The biological function and clinical significance of STIL in osteosarcoma.
| ||
J Cell Physiol Primary cilium participates in radiation-induced bystander effects through TGF-β1 signaling
| ||
