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验证数据展示

  • WB analysis using 83484-2-RR (same clone as 83484-2-PBS)

    WB analysis using 83484-2-RR (same clone as 83484-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83484-2-RR (STEAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83484-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83484-2-RR (same clone as 83484-2-PBS)

    FC experiment of HeLa using 83484-2-RR (same clone as 83484-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug STEAP3 Recombinant antibody (83484-2-RR, Clone:1N5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83484-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83484-2-RR

    Affinity and Kinetic Characterization of 83484-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83484-2-RR against Human STEAP3 were performed. The affinity constant is 0.89 nM.

STEAP3 Recombinant monoclonal antibody, PBS Only

STEAP3 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83484-2-PBS

产品说明书

CloneNo. 1N5

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, FC (Intra), ELISA

别名

1N5, dudlin 2, Dudulin 2, Dudulin-2, EC:1.16.1.-

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy STEAP3 Ready-To-Use IHC Kit. STEAP3 primary antibody included.

产品信息

83484-2-PBS targets STEAP3 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag29528

Product name: Recombinant human STEAP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 60-130 aa of BC042150

Sequence: NPKRTARLFPSAAQVTFQEEAVSSPEVIFVAVFREHYSSLCSLSDQLAGKILVDVSNPTEQEHLQHRESNA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 STEAP family member 3
别名 1N5, dudlin 2, Dudulin 2, Dudulin-2, EC:1.16.1.-
计算分子量 488 aa, 55 kDa
观测分子量55 kDa
GenBank蛋白编号BC042150
基因名称 STEAP3
Gene ID (NCBI) 55240
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ658P3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STEAP3 (Six-Transmembrane Epithelial Antigen of Prostate 3) is also named as TSAP6, Dudulin-2 and pHyde, and belongs to the STEAP family. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).

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