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验证数据展示

  • WB analysis using 28478-1-AP (same clone as 28478-1-PBS)

    WB analysis using 28478-1-AP (same clone as 28478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28478-1-AP (STEAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28478-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 28478-1-AP (same clone as 28478-1-PBS)

    IHC staining of human appendicitis using 28478-1-AP (same clone as 28478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28478-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STEAP3 antibody included
  • IHC staining of human appendicitis using 28478-1-AP (same clone as 28478-1-PBS)

    IHC staining of human appendicitis using 28478-1-AP (same clone as 28478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28478-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STEAP3 antibody included
  • IHC staining of human urothelial carcinoma using 28478-1-AP (same clone as 28478-1-PBS)

    IHC staining of human urothelial carcinoma using 28478-1-AP (same clone as 28478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28478-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STEAP3 antibody included
  • IHC staining of human urothelial carcinoma using 28478-1-AP (same clone as 28478-1-PBS)

    IHC staining of human urothelial carcinoma using 28478-1-AP (same clone as 28478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28478-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STEAP3 antibody included
  • IHC staining of mouse liver using 28478-1-AP (same clone as 28478-1-PBS)

    IHC staining of mouse liver using 28478-1-AP (same clone as 28478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28478-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STEAP3 antibody included
  • IF Staining of HepG2 using 28478-1-AP (same clone as 28478-1-PBS)

    IF Staining of HepG2 using 28478-1-AP (same clone as 28478-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STEAP3 antibody (28478-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28478-1-PBS in a different storage buffer formulation.

STEAP3 Polyclonal antibody, PBS Only

STEAP3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28478-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy STEAP3 Ready-To-Use IHC Kit. STEAP3 primary antibody included.

产品信息

28478-1-PBS targets STEAP3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29528

Product name: Recombinant human STEAP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 60-130 aa of BC042150

Sequence: NPKRTARLFPSAAQVTFQEEAVSSPEVIFVAVFREHYSSLCSLSDQLAGKILVDVSNPTEQEHLQHRESNA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 STEAP family member 3
别名
计算分子量 488 aa, 55 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC042150
基因名称 STEAP3
Gene ID (NCBI) 55240
RRIDAB_3086055
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ658P3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STEAP3 (Six-Transmembrane Epithelial Antigen of Prostate 3) is also named as TSAP6, Dudulin-2 and pHyde, and belongs to the STEAP family. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).

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