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STAT3 Recombinant antibody

STAT3 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3H1

Cat No : 80149-1-RR

Print datasheet

Synonyms

Acute phase response factor, APRF, FLJ20882, HIES, STAT3

验证数据展示

  • WB analysis using 80149-1-RR

    WB analysis using 80149-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80149-1-RR (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 80149-1-RR

    WB analysis using 80149-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80149-1-RR (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 80149-1-RR

    WB analysis of HeLa using 80149-1-RR

    WB result of STAT3 antibody (80149-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT3 transfected HeLa cells.

经过测试的应用

Positive WB detected inPC-12 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, Neuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy STAT3 Ready-To-Use IHC Kit. STAT3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80149-1-RR targets STAT3 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, ELISA
Tested Reactivity Human, Mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Synonyms Acute phase response factor, APRF, FLJ20882, HIES, STAT3
Calculated Molecular Weight 770 aa, 88 kDa
Observed Molecular Weight88 kDa
GenBank Accession NumberBC000627
Gene Symbol STAT3
Gene ID (NCBI) 6774
RRIDAB_2918869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP40763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3.

实验方案

Product Specific Protocols
WB protocol for STAT3 antibody 80149-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols