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验证数据展示

  • IF Staining of mouse testis using CL594-67130

    IF Staining of mouse testis using CL594-67130

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 STAR antibody (CL594-67130, Clone: 1G6E6 ) at dilution of 1:200.

  • IF Staining of mouse testis using CL594-67130

    IF Staining of mouse testis using CL594-67130

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 STAR antibody (CL594-67130, Clone: 1G6E6 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-67130

    FC experiment of HepG2 using CL594-67130

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human STAR (CL594-67130, Clone:1G6E6) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite®594-conjugated STAR Monoclonal antibody

STAR Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL594-67130

产品说明书

CloneNo. 1G6E6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF-P, FC (Intra)

别名

1G6E6, STARD1, START domain-containing protein 1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse testis tissue
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67130-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67130 targets STAR in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag28698

Product name: Recombinant human STAR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-285 aa of BC010550

Sequence: MLLATFKLCAGSSYRHMRNMKGLRQQAVMAISQELNRRALGGPTPSTWINQVRRRSSLLGSRLEETLYSDQELAYLQQGEEAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLAGMATDFGNMPEQKGVIRAEHGPTCMVLHPLAGSPSKTKLTWLLSIDLKGWLPKSIINQVLSQTQVDFANHLRKRLESHPASEARC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 steroidogenic acute regulatory protein
别名 1G6E6, STARD1, START domain-containing protein 1
计算分子量 285 aa, 32 kDa
观测分子量 28-32 kDa
GenBank蛋白编号BC010550
基因名称 STAR
Gene ID (NCBI) 6770
RRIDAB_2934758
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP49675
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue

实验方案

Product Specific Protocols
FC protocol for CL594 STAR antibody CL594-67130Download protocol
IF protocol for CL594 STAR antibody CL594-67130Download protocol
Standard Protocols
Click here to view our Standard Protocols
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