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CoraLite®594-conjugated STAR Monoclonal antibody

STAR Monoclonal Antibody for FC (Intra), IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, pig, mouse

Applications

FC (Intra), IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G6E6

Cat No : CL594-67130

Print datasheet

Synonyms

STAR, STARD1

验证数据展示

  • IF Staining of mouse testis using CL594-67130

    IF Staining of mouse testis using CL594-67130

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 STAR antibody (CL594-67130, Clone: 1G6E6 ) at dilution of 1:200.

  • IF Staining of mouse testis using CL594-67130

    IF Staining of mouse testis using CL594-67130

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 STAR antibody (CL594-67130, Clone: 1G6E6 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-67130

    FC experiment of HepG2 using CL594-67130

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human STAR (CL594-67130, Clone:1G6E6) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inmouse testis tissue
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67130-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67130 targets STAR in IF, FC (Intra) applications and shows reactivity with Human, rat, pig, mouse samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity Human, rat, pig, mouse
Immunogen STAR fusion protein Ag28698 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name steroidogenic acute regulatory protein
Synonyms STAR, STARD1
Calculated Molecular Weight 285 aa, 32 kDa
Observed Molecular Weight 28-32 kDa
GenBank Accession NumberBC010550
Gene Symbol STAR
Gene ID (NCBI) 6770
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49675
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue

实验方案

Product Specific Protocols
IF protocol for CL594 STAR antibody CL594-67130Download protocol
FC protocol for CL594 STAR antibody CL594-67130Download protocol
Standard Protocols
Click here to view our Standard Protocols