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验证数据展示

  • WB analysis using 86601-3-RR (same clone as 86601-3-PBS)

    WB analysis using 86601-3-RR (same clone as 86601-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86601-3-RR (STAMBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86601-3-PBS in a different storage buffer formulation.

  • WB analysis using 86601-3-RR (same clone as 86601-3-PBS)

    WB analysis using 86601-3-RR (same clone as 86601-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86601-3-RR (STAMBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86601-3-PBS in a different storage buffer formulation.

  • WB analysis of rat brain using 86601-3-RR (same clone as 86601-3-PBS)

    WB analysis of rat brain using 86601-3-RR (same clone as 86601-3-PBS)

    Rat brain tissue was subjected to SDS PAGE followed by western blot with 86601-3-RR (STAMBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86601-3-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 86601-3-RR (same clone as 86601-3-PBS)

    IF Staining of K-562 using 86601-3-RR (same clone as 86601-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using STAMBP antibody (86601-3-RR, Clone: 251545B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86601-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86601-3-RR

    Affinity and Kinetic Characterization of 86601-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86601-3-RR against Human STAMBP were performed. The affinity constant is 15.2 pM.

STAMBP Recombinant monoclonal antibody, PBS Only

STAMBP Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86601-3-PBS

产品说明书

CloneNo. 251545B3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

AMSH, EC:3.4.19.-, Endosome-associated ubiquitin isopeptidase, STAM binding protein, STAM-binding protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86601-3-PBS targets STAMBP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1895

Product name: Recombinant human STAMBP protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 124-424 aa of BC007682

Sequence: EKKKEAEELARNMAIQQELEKEKQRVAQQKQQQLEQEQFHAFEEMIRNQELEKERLKIVQEFGKVDPGLGGPLVPDLEKPSLDVFPTLTVSSIQPSDCHTTVRPAKPPVVDRSLKPGALSNSESIPTIDGLRHVVVPGRLCPQFLQLASANTARGVETCGILCGKLMRNEFTITHVLIPKQSAGSDYCNTENEEELFLIQDQQGLITLGWIHTHPTQTAFLSSVDLHTHCSYQMMLPESVAIVCSPKFQETGFFKLTDHGLEEISSCRQKGFHPHSKDPPLFCSCSHVTVVDRAVTITDLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 STAM binding protein
别名 AMSH, EC:3.4.19.-, Endosome-associated ubiquitin isopeptidase, STAM binding protein, STAM-binding protein
计算分子量 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC007682
基因名称 STAMBP
Gene ID (NCBI) 10617
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95630
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STAM-binding protein (STAMBP) is also named AMSH. STAMBP, a member of the Jab1/MPN metalloenzyme family of deubiquitinating enzymes (DUBs), specifically cleaves K63-linked polyubiquitination chains from substrates (PMID: 15314065, PMID: 26601948). STAMBP is implicated in the endosome-lysosome pathway and shuttles ubiquitinated substrate proteins to lysosomes for degradation (PMID: 10383417, PMID: 24151880). STAMBP regulates protein degradation by binding to the ESCRT-0 protein STAM and the charged multivesicular body proteins of the ESCRT-III complex (PMID: 20159979). STAMBP is implicated in the inflammatory response by promoting the deubiquitination and stabilization of NALP7 and increasing the activity of inflammasomes (PMID: 28492230). STAMBP expression was increased in the cytoplasm of tumor cells from LUAD patients. STAMBP overexpression promoted cell migration and invasion, whereas STAMBP knockdown attenuated these processes in LUAD cells after epidermal growth factor treatment (PMID: 3410245).

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