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验证数据展示

  • WB analysis of Jurkat using 19837-1-AP

    WB analysis of Jurkat using 19837-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 19837-1-AP

    IHC staining of human cervical cancer using 19837-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19837-1-AP (STAG2 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with STAG2 antibody included
  • IHC staining of human cervical cancer using 19837-1-AP

    IHC staining of human cervical cancer using 19837-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19837-1-AP (STAG2 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with STAG2 antibody included
  • IF Staining of HEK-293 using 19837-1-AP

    IF Staining of HEK-293 using 19837-1-AP

    Immunofluorescent analysis of 293 cells, using STAG2 antibody 19837-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of MCF-7 using 19837-1-AP

    IP experiment of MCF-7 using 19837-1-AP

    IP result of anti-STAG2 (IP:19837-1-AP, 4ug; Detection:19837-1-AP 1:3000) with MCF-7 cells lysate 2120 ug.

  • IP experiment of U-251 using 19837-1-AP

    IP experiment of U-251 using 19837-1-AP

    IP result of anti-STAG2 (IP:19837-1-AP, 4ug; Detection:19837-1-AP 1:3000) with U-251 cells lysate 3320 ug.

  • IF Staining of HEK-293 using 19837-1-AP

    IF Staining of HEK-293 using 19837-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STAG2 antibody (19837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

STAG2 Polyclonal antibody

STAG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 19837-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Stromal antigen 2, SA2, SA 2, Cohesin subunit SA-2, Cohesin subunit SA 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, K-562 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inU-251 cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy STAG2 Ready-To-Use IHC Kit. STAG2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19837-1-AP targets STAG2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag13825

Product name: Recombinant human STAG2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1047-1172 aa of BC001765

Sequence: SLLAGGDDDTMSVISGISSRGSTVRSKKSKPSTGKRKVVEGMQLSLTEESSSSDSMWLSREQTLHTPVMMQTPQLTSTIMREPKRLRPEDSFMSVYPMQTEHHQTPLDYNRRGTSLMEDDEEPIVE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 stromal antigen 2
别名 Stromal antigen 2, SA2, SA 2, Cohesin subunit SA-2, Cohesin subunit SA 2
计算分子量 1231 aa, 141 kDa
观测分子量 130-141 kDa
GenBank蛋白编号BC001765
基因名称 STAG2
Gene ID (NCBI) 10735
RRIDAB_10666847
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N3U4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAG2, also named as SA2, is a component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex may play a role in spindle pole assembly during mitosis. Mutations in STAG2 appear to be a first step in the transformation of a normal cell into a cancer cell.

实验方案

Product Specific Protocols
IF protocol for STAG2 antibody 19837-1-APDownload protocol
IHC protocol for STAG2 antibody 19837-1-APDownload protocol
IP protocol for STAG2 antibody 19837-1-APDownload protocol
WB protocol for STAG2 antibody 19837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Commun (Lond)

Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1-mediated MAPK signaling.

Authors - Luwei He
View Article
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