STAG2 Polyclonal antibody

STAG2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 19837-1-AP

Print datasheet

Synonyms

Cohesin subunit SA 2, SA 2, SA2, SCC3 homolog 2, STAG2, stromal antigen 2



经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, K-562 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inU-251 cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19837-1-AP targets STAG2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen STAG2 fusion protein Ag13825 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name stromal antigen 2
Synonyms Cohesin subunit SA 2, SA 2, SA2, SCC3 homolog 2, STAG2, stromal antigen 2
Calculated Molecular Weight 1231 aa, 141 kDa
Observed Molecular Weight 130-141 kDa
GenBank Accession NumberBC001765
Gene Symbol STAG2
Gene ID (NCBI) 10735
RRIDAB_10666847
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N3U4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAG2, also named as SA2, is a component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex may play a role in spindle pole assembly during mitosis. Mutations in STAG2 appear to be a first step in the transformation of a normal cell into a cancer cell.

实验方案

Product Specific Protocols
WB protocol for STAG2 antibody 19837-1-APDownload protocol
IHC protocol for STAG2 antibody 19837-1-APDownload protocol
IF protocol for STAG2 antibody 19837-1-APDownload protocol
IP protocol for STAG2 antibody 19837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Commun (Lond)

Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1-mediated MAPK signaling.

Authors - Luwei He