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验证数据展示

  • WB analysis using 24918-1-AP (same clone as 24918-1-PBS)

    WB analysis using 24918-1-AP (same clone as 24918-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24918-1-AP (ST8SIA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24918-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin using 24918-1-AP (same clone as 24918-1-PBS)

    IHC staining of human skin using 24918-1-AP (same clone as 24918-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24918-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin using 24918-1-AP (same clone as 24918-1-PBS)

    IHC staining of human skin using 24918-1-AP (same clone as 24918-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24918-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 24918-1-AP (same clone as 24918-1-PBS)

    IHC staining of mouse brain using 24918-1-AP (same clone as 24918-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24918-1-AP (ST8SIA1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24918-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ST8SIA1 Polyclonal antibody, PBS Only

ST8SIA1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24918-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Alpha-2,8-sialyltransferase 8A, Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase, EC:2.4.3.8, Sialyltransferase St8Sia I, SIAT8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

24918-1-PBS targets ST8SIA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20429

Product name: Recombinant human ST8SIA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 39-136 aa of BC126162

Sequence: VLCWLYIFPVYRLPNEKEIVQGVLQQGTAWRRNQTAARAFRKQMEDCCDPAHLFAMTKMNSPMGKSMWYDGEFLYSFTIDNSTYSLFPQATPFQLPLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
别名 Alpha-2,8-sialyltransferase 8A, Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase, EC:2.4.3.8, Sialyltransferase St8Sia I, SIAT8
计算分子量 356 aa, 41 kDa
观测分子量 43 kDa
GenBank蛋白编号BC126162
基因名称 ST8SIA1
Gene ID (NCBI) 6489
RRIDAB_2879796
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92185
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ST8SIA1 is a member of the ST family involved in the production of gangliosides. ST8SIA1 is strongly expressed in melanoma cell lines, adult and fetal brain, and to a lesser extent in adult and fetal lung. The abnormal expression of ST8SIA1 has been demonstrated to influence the metastasis of triple-negative breast cancer. (PMID: 34429775)

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