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验证数据展示

  • WB analysis of HepG2 using 14355-1-AP

    WB analysis of HepG2 using 14355-1-AP

    WB result of ST6GAL1 antibody (14355-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ST6GAL1 transfected HepG2 cells.

  • WB analysis using 14355-1-AP

    WB analysis using 14355-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 14355-1-AP

    IP experiment of Raji using 14355-1-AP

    IP result of anti-ST6GAL1 (IP:14355-1-AP, 4ug; Detection:14355-1-AP 1:3000) with Raji cells lysate 1800 ug.

  • IHC staining of human colon cancer using 14355-1-AP

    IHC staining of human colon cancer using 14355-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14355-1-AP (ST6GAL1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ST6GAL1 antibody included
  • Featured Product
  • KD/KO Validated

ST6GAL1 Polyclonal antibody

ST6GAL1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14355-1-AP

产品说明书

引用文献(18)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IP, ELISA and More (1)

别名

Alpha 2,6-ST 1, B-cell antigen CD75, Beta-galactoside alpha-2,6-sialyltransferase 1, CD75, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HuH-7 cells, Jurkat cells, mouse spleen tissue, U-937 cells, L02 cells
Positive IP detected inRaji cells
Positive IHC detected inhuman colon cancer tissue, human ovary cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ST6GAL1 Ready-To-Use IHC Kit. ST6GAL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14355-1-AP targets ST6GAL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag5705

Product name: Recombinant human ST6GAL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 60-406 aa of BC040009

Sequence: SQSVSSSSTQDPHRGRQTLGSLRGLAKAKPEASFQVWNKDSSSKNLIPRLQKIWKNYLSMNKYKVSYKGPGPGIKFSAEALRCHLRDHVNVSMVEVTDFPFNTSEWEGYLPKESIRTKAGPWGRCAVVSSAGSLKSSQLGREIDDHDAVLRFNGAPTANFQQDVGTKTTIRLMNSQLVTTEKRFLKDSLYNEGILIVWDPSVYHSDIPKWYQNPDYNFFNNYKTYRKLHPNQPFYILKPQMPWELWDILQEISPEEIQPNPPSSGMLGIIIMMTLCDQVDIYEFLPSKRKTDVCYYYQKFFDSACTMGAYHPLLYEKNLVKHLNQGTDEDIYLLGKATLPGFRTIHC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
别名 Alpha 2,6-ST 1, B-cell antigen CD75, Beta-galactoside alpha-2,6-sialyltransferase 1, CD75, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
计算分子量 47 kDa
观测分子量 43-45 kDa, 50-70 kDa
GenBank蛋白编号BC040009
基因名称 ST6GAL1
Gene ID (NCBI) 6480
RRIDAB_2194422
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15907
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ST6GAL1 (β-galactoside α-2-6 sialyl transferase1; also known as ST6N or CD75) is a sialyltransferase mediating the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment. It is principally expressed in liver, placenta, and skeletal muscle. ST6GAL1 undergoes proteolytic process to generate soluble form from membrane form. Western blot analysis of human liver using this antibody detects both isoforms between 43-50 kDa. Higher molecular weight of bands around 50-70 kDa can also be observed with glycosylation modification. (PMID: 15049997, 23358684)

实验方案

Product Specific Protocols
IHC protocol for ST6GAL1 antibody 14355-1-APDownload protocol
IP protocol for ST6GAL1 antibody 14355-1-APDownload protocol
WB protocol for ST6GAL1 antibody 14355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IF

Immunity

Immune checkpoint therapy-elicited sialylation of IgG antibodies impairs antitumorigenic type I interferon responses in hepatocellular carcinoma

Authors - Rui-Qi Wu
View Article
humanWB,IHC

J Exp Clin Cancer Res

Long noncoding RNA TINCR facilitates hepatocellular carcinoma progression and dampens chemosensitivity to oxaliplatin by regulating the miR-195-3p/ST6GAL1/NF-κB pathway

Authors - Jie Mei
  • KD Validated
View Article

Adv Sci (Weinh)

A Glycoproteome Data Mining Strategy for Characterizing Structural Features of Altered Glycans with Thymic Involution

Authors - Zhida Zhang
View Article
humanWB,IHC

Cell Death Dis

Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway.

Authors - Yang Han
View Article
mouseIF

J Neuroinflammation

St6gal1-mediated sialylation protects retinal ganglion cells by restraining microglial phagocytosis.

Authors - Liyan Liu
View Article

Adv Healthc Mater

Nanointegrative Glycoengineering-Activated Necroptosis of Triple Negative Breast Cancer Stem Cells Enables Self-Amplifiable Immunotherapy for Systemic Tumor Rejection

Authors - Youbo Zhao
View Article
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