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ST6GAL1 Polyclonal antibody
ST6GAL1 Polyclonal Antibody for WB, IP, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, FC, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Ramos cells, Jurkat cells, mouse liver tissue, mouse spleen tissue, Raji cells, HepG2 cells, HuH-7 cells, U-937 cells, rat liver tissue |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human skin tissue, human colon cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | Raji cells |
Planning an IHC experiment? We recommend our IHCeasy ST6GAL1 Ready-To-Use IHC Kit. ST6GAL1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14355-1-AP targets ST6GAL1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, FC, IHC, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | ST6GAL1 fusion protein Ag5705 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 |
| Synonyms | Alpha 2,6 ST 1, B cell antigen CD75, CD75, Sialyltransferase 1, SIAT1, ST6Gal I, ST6GAL1, ST6GalI, ST6N |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 43-45 kDa, 50-70 kDa |
| GenBank Accession Number | BC040009 |
| Gene Symbol | ST6GAL1 |
| Gene ID (NCBI) | 6480 |
| RRID | AB_2194422 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15907 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ST6GAL1 (β-galactoside α-2-6 sialyl transferase1; also known as ST6N or CD75) is a sialyltransferase mediating the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment. It is principally expressed in liver, placenta, and skeletal muscle. ST6GAL1 undergoes proteolytic process to generate soluble form from membrane form. Western blot analysis of human liver using this antibody detects both isoforms between 43-50 kDa. Higher molecular weight of bands around 50-70 kDa can also be observed with glycosylation modification. (PMID: 15049997, 23358684)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ST6GAL1 antibody 14355-1-AP | Download protocol |
| IHC protocol for ST6GAL1 antibody 14355-1-AP | Download protocol |
| IP protocol for ST6GAL1 antibody 14355-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Immune checkpoint therapy-elicited sialylation of IgG antibodies impairs antitumorigenic type I interferon responses in hepatocellular carcinoma | ||
Int J Cancer Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.
| ||
Mol Cell Proteomics Integrated Systems Analysis of the Murine and Human Pancreatic Cancer Glycomes Reveals a Tumor-Promoting Role for ST6GAL1
| ||
Front Oncol High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.
| ||
Am J Cancer Res α1,6-Fucosyltransferase (FUT8) regulates the cancer-promoting capacity of cancer-associated fibroblasts (CAFs) by modifying EGFR core fucosylation (CF) in non-small cell lung cancer (NSCLC). | ||
Int J Food Sci Nutr Ellagic acid alleviates TNBS-induced intestinal barrier dysfunction by regulating mucin secretion and maintaining tight junction integrity in rats |
