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验证数据展示

  • WB analysis using 84673-5-RR

    WB analysis using 84673-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84673-5-RR (SSRP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 84673-5-RR

    WB analysis of HEK-293 using 84673-5-RR

    WB result of SSRP1 antibody (84673-5-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SSRP1 transfected HEK-293 cells.

  • FC experiment of HeLa using 84673-5-RR

    FC experiment of HeLa using 84673-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of SH-SY5Y using 84673-5-RR

    FC experiment of SH-SY5Y using 84673-5-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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SSRP1 Recombinant monoclonal antibody

SSRP1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84673-5-RR

产品说明书

CloneNo. 242102H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

242102H8, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, FACT

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, Jurkat cells
Positive FC (Intra) detected inHeLa cells, SH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy SSRP1 Ready-To-Use IHC Kit. SSRP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84673-5-RR targets SSRP1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8318

Product name: Recombinant human SSRP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 361-709 aa of BC005116

Sequence: YVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNAKKLNIKNRGLKEGMNPSYDEYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEEDVAEEFDSNASASSSSNEGDSDRDEKKRKQLKKAKMAKDRKSRKKPVEVKKGKDPNAPKRPMSAYMLWLNASREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARRDYEKAMKEYEGGRGESSKRDKSKKKKKVKVKMEKKSTPSRGSSSKSSSRQLSESFKSKEFVSSDESSSGENKSKKKRRRSEDSEEEELASTPPSSEDSASGSDE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 structure specific recognition protein 1
别名 242102H8, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, FACT
计算分子量 81 kDa
观测分子量86 kDa
GenBank蛋白编号BC005116
基因名称 SSRP1
Gene ID (NCBI) 6749
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ08945
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SSRP1 antibody 84673-5-RRDownload protocol
FC protocol for SSRP1 antibody 84673-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols