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  • KD/KO Validated

SRX1 Polyclonal antibody

SRX1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14273-1-AP

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Synonyms

C20orf139, SRX, SRX1, SRXN1, Sulfiredoxin 1, YKL086W



经过测试的应用

Positive WB detected inA549 cells, mouse liver tissue, Transfected HEK-293 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14273-1-AP targets SRX1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen SRX1 fusion protein Ag5613 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sulfiredoxin 1 homolog (S. cerevisiae)
Synonyms C20orf139, SRX, SRX1, SRXN1, Sulfiredoxin 1, YKL086W
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC047707
Gene Symbol SRXN1
Gene ID (NCBI) 140809
RRIDAB_2195030
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYN0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SRX1 antibody 14273-1-APDownload protocol
IHC protocol for SRX1 antibody 14273-1-APDownload protocol
IP protocol for SRX1 antibody 14273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Nat Commun

Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle.

Authors - John T Olthoff
humanWB,IHC

Proc Natl Acad Sci U S A

Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling.

Authors - Wei Qiou Q
humanIHC

Cancer Res

Genetic polymorphisms and protein expression of NRF2 and Sulfiredoxin predict survival outcomes in breast cancer.

Authors - Hartikainen Jaana M JM
humanWB

Oncogene

PMID: 28504720

Authors - E-J Choi
human,mouseWB,IHC

Cancer Lett

Nrf2-activated expression of sulfiredoxin contributes to urethane-induced lung tumorigenesis.

Authors - Murli Mishra
  • KD Validated
mouseWB,IHC

Carcinogenesis

Tumor Promoter-induced Sulfiredoxin Is Required for Mouse Skin Tumorigenesis.

Authors - Lisha Wu