验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse liver tissue, Transfected HEK-293 cells, HepG2 cells, U-251 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14273-1-AP targets SRX1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5613 Product name: Recombinant human SRX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-137 aa of BC047707 Sequence: MGLRAGGTLGRAGAGRGAPEGPGPSGGAQGGSIHSGRIAAVHNVPLSVLIRPLPSVLDPAKVQSLVDTIREDPDSVPPIDVLWIKGAQGGDYFYSFGGCHRYAAYQQLQRETIPAKLVQSTLSDLRVYLGASTPDLQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sulfiredoxin 1 homolog (S. cerevisiae) |
| 别名 | C20orf139, SRX, SRX1, SRXN1, Sulfiredoxin 1, YKL086W |
| 计算分子量 | 14 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC047707 |
| 基因名称 | SRXN1 |
| Gene ID (NCBI) | 140809 |
| RRID | AB_2195030 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BYN0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SRX1 antibody 14273-1-AP | Download protocol |
| IP protocol for SRX1 antibody 14273-1-AP | Download protocol |
| WB protocol for SRX1 antibody 14273-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle. | ||
Proc Natl Acad Sci U S A Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling. | ||
Cancer Res Genetic polymorphisms and protein expression of NRF2 and Sulfiredoxin predict survival outcomes in breast cancer. | ||
Cancer Lett Nrf2-activated expression of sulfiredoxin contributes to urethane-induced lung tumorigenesis.
| ||
Carcinogenesis Tumor Promoter-induced Sulfiredoxin Is Required for Mouse Skin Tumorigenesis. |
