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验证数据展示

  • WB analysis using 20353-1-AP (same clone as 20353-1-PBS)

    WB analysis using 20353-1-AP (same clone as 20353-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 20353-1-AP (same clone as 20353-1-PBS)

    WB analysis of NIH/3T3 using 20353-1-AP (same clone as 20353-1-PBS)

    WB result of SRRT antibody (20353-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRRT transfected NIH/3T3 cells. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

  • WB analysis using 20353-1-AP (same clone as 20353-1-PBS)

    WB analysis using 20353-1-AP (same clone as 20353-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 20353-1-AP (same clone as 20353-1-PBS)

    IF Staining of MCF-7 using 20353-1-AP (same clone as 20353-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SRRT antibody (20353-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 20353-1-AP (same clone as 20353-1-PBS)

    IP experiment of Jurkat using 20353-1-AP (same clone as 20353-1-PBS)

    IP result of anti-SRRT (IP:20353-1-AP, 4ug; Detection:20353-1-AP 1:30000) with Jurkat cells lysate 1440 ug. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SRRT Polyclonal antibody, PBS Only

SRRT Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 20353-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, Indirect ELISA

别名

serrate, ASR2, Arsenite-resistance protein 2, Arsenite resistance protein 2, ARS2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SRRT Ready-To-Use IHC Kit. SRRT primary antibody included.

产品信息

20353-1-PBS targets SRRT in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14196

Product name: Recombinant human SRRT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 527-876 aa of BC000082

Sequence: LIHTLDDRTQLWASEPGTPPLPTSLPSQNPILKNITDYLIEEVSAEEEELLGSSGGAPPEEPPKEGNPAEINVERDEKLIKVLDKLLLYLRIVHSLDYYNTCEYPNEDEMPNRCGIIHVRGPMPPNRISHGEVLEWQKTFEEKLTPLLSVRESLSEEEAQKMGRKDPEQEVEKFVTSNTQELGKDKWLCPLSGKKFKGPEFVRKHIFNKHAEKIEEVKKEVAFFNNFLTDAKRPALPEIKPAQPPGPAQILPPGLTPGLPYPHQTPQGLMPYGQPRPPILGYGAGAVRPAVPTGGPPYPHAPYGAGRGNYDAFRGQGGYPGKPRNRMVRGDPRAIVEYRDLDAPDDVDFF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serrate RNA effector molecule homolog (Arabidopsis)
别名 serrate, ASR2, Arsenite-resistance protein 2, Arsenite resistance protein 2, ARS2
计算分子量 876 aa, 101 kDa
观测分子量 120 kDa
GenBank蛋白编号BC000082
基因名称 SRRT
Gene ID (NCBI) 51593
RRIDAB_3085603
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXP5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SRRT (Serrate RNA effector molecule homolog) is also named as ARS2 (Arsenite-resistance protein 2). ARS2/SRRT is an essential eukaryotic protein that has emerged as a critical factor in the sorting of functional from non-functional RNA polymerase II (Pol II) transcripts (PMID: 34060620). SRRT protein is a key component of the nuclear cap-binding complex (CBC), which allows it to modulate the expression of various miRNAs. The SRRT protein is widely expressed throughout the eukaryotes, but not in budding yeast. SRRT has been shown to play a vital role in stem-cell renewal, proliferation, hematopoiesis, and RNA-mediated gene silencing and regulation of miRNA biogenesis (PMID: 37345203). SRRT is involved in cellular proliferation and colony formation (tumorigenicity). Similarly, high SRRT expression has been found in cholangiocarcinoma. ARS2 showed ubiquitous expression, with the highest levels in mouse testes, heart, and liver and human heart and skeletal muscle (PMID: 18086880).

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