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SRRT Polyclonal antibody
SRRT Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, NIH/3T3 cells, HeLa cells, HepG2 cells, K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy SRRT Ready-To-Use IHC Kit. SRRT primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:16000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20353-1-AP targets SRRT in WB, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, ELISA |
Tested Reactivity | human, mouse |
Immunogen | SRRT fusion protein Ag14196 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | serrate RNA effector molecule homolog (Arabidopsis) |
Synonyms | ARS2, Arsenite resistance protein 2, ASR2, serrate, SRRT |
Calculated Molecular Weight | 876 aa, 101 kDa |
Observed Molecular Weight | 120 kDa |
GenBank Accession Number | BC000082 |
Gene Symbol | SRRT |
Gene ID (NCBI) | 51593 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9BXP5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SRRT (Serrate RNA effector molecule homolog) is also named as ARS2 (Arsenite-resistance protein 2). ARS2/SRRT is an essential eukaryotic protein that has emerged as a critical factor in the sorting of functional from non-functional RNA polymerase II (Pol II) transcripts (PMID: 34060620). SRRT protein is a key component of the nuclear cap-binding complex (CBC), which allows it to modulate the expression of various miRNAs. The SRRT protein is widely expressed throughout the eukaryotes, but not in budding yeast. SRRT has been shown to play a vital role in stem-cell renewal, proliferation, hematopoiesis, and RNA-mediated gene silencing and regulation of miRNA biogenesis (PMID: 37345203). SRRT is involved in cellular proliferation and colony formation (tumorigenicity). Similarly, high SRRT expression has been found in cholangiocarcinoma. ARS2 showed ubiquitous expression, with the highest levels in mouse testes, heart, and liver and human heart and skeletal muscle (PMID: 18086880).
实验方案
Product Specific Protocols | |
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WB protocol for SRRT antibody 20353-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |