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验证数据展示

  • WB analysis using 14636-1-AP

    WB analysis using 14636-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14636-1-AP (SRPRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 14636-1-AP

    IHC staining of human liver using 14636-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14636-1-AP (SRPRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 14636-1-AP

    IHC staining of human liver using 14636-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14636-1-AP (SRPRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SRPRB Polyclonal antibody

SRPRB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14636-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

APMCF1, Protein APMCF1, SR beta, SRPRB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, HepG2 cells, human liver tissue, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14636-1-AP targets SRPRB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag6215

Product name: Recombinant human SRPRB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-271 aa of BC065299

Sequence: MASADSRRVADGGGAGGTFQPYLDTLRQELQQTDPTLLSVVVAVLAVLLTLVFWKLIRSRRSSQRAVLLVGLCDSGKTLLFVRLLTGLYRDTQTSITDSCAVYRVNNNRGNSLTLIDLPGHESLRLQFLERFKSSARAIVFVVDSAAFQREVKDVAEFLYQVLIDSMGLKNTPSFLIACNKQDIAMAKSAKLIQQQLEKELNTLRVTRSAAPSTLDSSSTAPAQLGKKGKEFEFSQLPLKVEFLECSAKGGRGDVGSADIQDLEKWLAKIA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal recognition particle receptor, B subunit
别名 APMCF1, Protein APMCF1, SR beta, SRPRB
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC065299
基因名称 SRPRB
Gene ID (NCBI) 58477
RRIDAB_2194868
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5M8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SRPRB antibody 14636-1-APDownload protocol
IHC protocol for SRPRB antibody 14636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

Signal recognition particle receptor-β (SR-β) coordinates cotranslational N-glycosylation

Authors - Chatchai Phoomak
View Article