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SRP9 Polyclonal antibody

SRP9 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11195-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

ALURBP, SRP9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, A2780 cells, HeLa cells, MCF-7 cells, SKOV-3 cells, mouse colon tissue, HuH-7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11195-1-AP targets SRP9 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 SRP9 fusion protein Ag1683 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal recognition particle 9kDa
别名 ALURBP, SRP9
计算分子量 10 kDa
观测分子量 10 kDa
GenBank蛋白编号BC015094
基因名称 SRP9
Gene ID (NCBI) 6726
RRIDAB_2239820
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49458
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signal recognition particle 9(SRP9) is component of the signal recognition particle (SRP), which is a ribonucleoprotein complex that mediates the targeting of proteins to the rough endoplasmic reticulum (ER). SRP9 form a heterodimer with SRP14, which involves in arrest of the elongation of the nescent chains during targeting to ensure efficient translocation of the preprotein.

实验方案

Product Specific Protocols
WB protocol for SRP9 antibody 11195-1-APDownload protocol
IHC protocol for SRP9 antibody 11195-1-APDownload protocol
IP protocol for SRP9 antibody 11195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer.

Authors - Barzin Y Nabet
  • KD Validated
humanWB,IP

Nucleic Acids Res

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.

Authors - Evan P Booy
WB

Nucleic Acids Res

The pioneer round of translation ensures proper targeting of ER and mitochondrial proteins.

Authors - Joori Park
humanWB,IP

Nucleic Acids Res

Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.

Authors - Piazzon Nathalie N
humanIHC

Int J Oncol

Significance of signal recognition particle 9 nuclear translocation: Implications for pancreatic cancer prognosis and functionality

Authors - Hiromichi Sato
humanWB

J Proteome Res

Proteomic expression analysis of surgical human colorectal cancer tissues: up-regulation of PSB7, PRDX1, and SRP9 and hypoxic adaptation in cancer.

Authors - Rho Jung-hyun JH
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