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验证数据展示

  • FC experiment of HeLa using CL488-67005

    FC experiment of HeLa using CL488-67005

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SRP54 (CL488-67005, Clone:1D6D1) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-67005

    IF Staining of HepG2 using CL488-67005

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 SRP54 antibody (CL488-67005, Clone: 1D6D1 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated SRP54 Monoclonal antibody

SRP54 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67005

产品说明书

CloneNo. 1D6D1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 1D6D1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67005-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67005 targets SRP54 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 SRP54 fusion protein Ag12166 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 signal recognition particle 54kDa
别名 Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 1D6D1
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC003389
基因名称 SRP54
Gene ID (NCBI) 6729
RRIDAB_3672934
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP61011
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SRP54 antibody CL488-67005Download protocol
FC protocol for CL Plus 488 SRP54 antibody CL488-67005Download protocol
Standard Protocols
Click here to view our Standard Protocols