• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84014-6-RR

    WB analysis using 84014-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 84014-6-RR

    IF Staining of HepG2 using 84014-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SRP54 antibody (84014-6-RR, Clone: 241125G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human placenta using 84014-6-RR

    IHC staining of human placenta using 84014-6-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84014-6-RR (SRP54 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84014-6-RR

    Affinity and Kinetic Characterization of 84014-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84014-6-RR against Human SRP54 were performed. The affinity constant is 4.24 nM.

SRP54 Recombinant antibody

SRP54 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84014-6-RR

产品说明书

CloneNo. 241125G1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 241125G1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, RAW 264.7 cells, HEK-293 cells, MCF-7 cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84014-6-RR targets SRP54 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SRP54 fusion protein Ag2327 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal recognition particle 54kDa
别名 Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 241125G1
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC003389
基因名称 SRP54
Gene ID (NCBI) 6729
RRIDAB_3671584
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP61011
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).

实验方案

Product Specific Protocols
WB protocol for SRP54 antibody 84014-6-RRDownload protocol
IHC protocol for SRP54 antibody 84014-6-RRDownload protocol
IF protocol for SRP54 antibody 84014-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols