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验证数据展示

  • WB analysis using 84014-5-RR (same clone as 84014-5-PBS)

    WB analysis using 84014-5-RR (same clone as 84014-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84014-5-RR (SRP54 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 84014-5-RR (same clone as 84014-5-PBS)

    WB analysis of MCF-7 using 84014-5-RR (same clone as 84014-5-PBS)

    WB result of SRP54 antibody (84014-5-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRP54 transfected MCF-7 cells. This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84014-5-RR (same clone as 84014-5-PBS)

    IF Staining of HeLa using 84014-5-RR (same clone as 84014-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SRP54 antibody (84014-5-RR, Clone: 241125D6 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84014-5-RR (same clone as 84014-5-PBS)

    FC experiment of HepG2 using 84014-5-RR (same clone as 84014-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Srp54 Recombinant Antibody (84014-5-RR, Clone:241125D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00955-4

    Sandwich ELISA standard curve of MP00955-4

    Sandwich ELISA standard curve of MP00955-4, Human SRP54 Recombinant Matched Antibody Pair - PBS only. 84014-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2327. 84014-5-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP00955-4

    Sample test of MP00955-4

    The mean SRP54 concentration was determined to be 124.39 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load.

  • Affinity and Kinetic Characterization of 84014-5-RR

    Affinity and Kinetic Characterization of 84014-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84014-5-RR against Human SRP54 were performed. The affinity constant is 0.287 nM.

  • Featured Product
  • KD/KO验证

SRP54 Recombinant antibody, PBS Only (Detector)

SRP54 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84014-5-PBS

产品说明书

CloneNo. 241125D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

别名

241125D6, EC:3.6.5.4, SCN8, Signal recognition particle subunit SRP54

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84014-5-PBS targets SRP54 as part of a matched antibody pair:

MP00955-4: 84014-2-PBS capture and 84014-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 SRP54 fusion protein Ag2327 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal recognition particle 54kDa
别名 241125D6, EC:3.6.5.4, SCN8, Signal recognition particle subunit SRP54
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC003389
基因名称 SRP54
Gene ID (NCBI) 6729
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP61011
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).