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CoraLite®594-conjugated SRD5A1 Monoclonal antibody

SRD5A1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D5D8

Cat No : CL594-66329

Print datasheet

Synonyms

S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1

验证数据展示

  • IF Staining of HeLa using CL594-66329

    IF Staining of HeLa using CL594-66329

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66329 (SRD5A1 antibody) at dilution of 1:100.

经过测试的应用

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66329-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66329 targets SRD5A1 in IF applications and shows reactivity with human samples.

Tested Applications IF/ICC
Tested Reactivity human
Immunogen SRD5A1 fusion protein Ag23351 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
Synonyms S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC007033
Gene Symbol SRD5A1
Gene ID (NCBI) 6715
RRIDAB_2883559
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18405
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRD5A1 is a minor component of the reductase activity in prostate although the gene was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant isozyme of steroid 5-alpha-reductase in the scalp and elsewhere in the skin.

实验方案

Product Specific Protocols
IF protocol for CL594 SRD5A1 antibody CL594-66329Download protocol
Standard Protocols
Click here to view our Standard Protocols