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验证数据展示

  • IP experiment of mouse brain using 24931-1-AP

    IP experiment of mouse brain using 24931-1-AP

    IP result of anti-SRBD1 (IP:24931-1-AP, 4ug; Detection:24931-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • IHC staining of human brain using 24931-1-AP

    IHC staining of human brain using 24931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24931-1-AP (SRBD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 24931-1-AP

    IHC staining of human brain using 24931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24931-1-AP (SRBD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 24931-1-AP

    WB analysis using 24931-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24931-1-AP (SRBD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

SRBD1 Polyclonal antibody

SRBD1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 24931-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

S1 RNA binding domain 1, SRBD1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24931-1-AP targets SRBD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SRBD1 fusion protein Ag21661 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S1 RNA binding domain 1
别名 S1 RNA binding domain 1, SRBD1
计算分子量 995 aa, 112 kDa
观测分子量 100-120 kDa
GenBank蛋白编号BC032538
基因名称 SRBD1
Gene ID (NCBI) 55133
RRIDAB_2879805
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N5C6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S1 RNA-binding domain-containing protein 1 (SRBD1) is a 995 amino acid protein, which contains one S1 motif domain. SRBD1 is a susceptibility gene for normal tension glaucoma (NTG) and may be involved in cell growth inhibition and apoptosis. Two isoforms of SRBD1 exist due to alternative splicing events, and the gene which encodes SRBD1 maps to human chromosome 2p21.

实验方案

Product Specific Protocols
WB protocol for SRBD1 antibody 24931-1-APDownload protocol
IHC protocol for SRBD1 antibody 24931-1-APDownload protocol
IP protocol for SRBD1 antibody 24931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols