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验证数据展示

  • FC experiment of MCF-7 using CL488-24655

    FC experiment of MCF-7 using CL488-24655

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Sra1 Polyclonal Antibody (CL488-24655)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of MCF-7 using CL488-24655

    IF Staining of MCF-7 using CL488-24655

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 SRA1 antibody (CL488-24655) at dilution of 1:200.

CoraLite® Plus 488-conjugated SRA1 Polyclonal antibody

SRA1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-24655

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

PP7684, SRAP, Steroid receptor RNA activator 1, Steroid receptor RNA activator protein

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SRA1 Ready-To-Use IHC Kit. SRA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24655-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24655 targets SRA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20082

Product name: Recombinant human SRA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-209 aa of BC067895

Sequence: GNKERGWNDPPQFSYGLQTQAGGPRRSLLTKRVAAPQDGSPRVPASETSPGPPPMGPPPPSSKAPRSPPVGSGPASGVEPTSFPVESEAVMEDVLRPLEQALEDCRGHTRKQVCDDISRRLALLQEQWAGGKLSIPVKKRMALLVQELSSHRWDAADDIHRSLMVDHVTEVSQWMVGVKRLIAEKRSLF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 steroid receptor RNA activator 1
别名 PP7684, SRAP, Steroid receptor RNA activator 1, Steroid receptor RNA activator protein
计算分子量 26 kDa
观测分子量 30 kDa, 33 kDa
GenBank蛋白编号BC067895
基因名称 SRA1
Gene ID (NCBI) 10011
RRIDAB_3672771
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HD15
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription. This polyclonal antibody recognizes endogenous SRA1 isoforms which migrate as a doublet on SDS-PAGE gels (PMID: 12565891; 23907597).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SRA1 antibody CL488-24655Download protocol
IF protocol for CL Plus 488 SRA1 antibody CL488-24655Download protocol
Standard Protocols
Click here to view our Standard Protocols
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