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CoraLite® Plus 488-conjugated SRA1 Polyclonal antibody

SRA1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-24655

Print datasheet

Synonyms

pp7684, SRA, SRA1, SRAP, STRAA1

验证数据展示

  • FC experiment of MCF-7 using CL488-24655

    FC experiment of MCF-7 using CL488-24655

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Sra1 Polyclonal Antibody (CL488-24655)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SRA1 Ready-To-Use IHC Kit. SRA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24655-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24655 targets SRA1 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse
Immunogen SRA1 fusion protein Ag20082 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name steroid receptor RNA activator 1
Synonyms pp7684, SRA, SRA1, SRAP, STRAA1
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 30 kDa, 33 kDa
GenBank Accession NumberBC067895
Gene Symbol SRA1
Gene ID (NCBI) 10011
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HD15
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription. This polyclonal antibody recognizes endogenous SRA1 isoforms which migrate as a doublet on SDS-PAGE gels (PMID: 12565891; 23907597).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SRA1 antibody CL488-24655Download protocol
Standard Protocols
Click here to view our Standard Protocols