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SPTLC3 Polyclonal antibody

SPTLC3 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16600-1-AP

Print datasheet

Synonyms

C20orf38, dJ718P11, LCB 3, LCB2b, Serine palmitoyltransferase 3, SPT 3, SPTLC2L, SPTLC3

验证数据展示

  • WB analysis using 16600-1-AP

    WB analysis using 16600-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16600-1-AP (SPTLC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16600-1-AP

    IF Staining of HepG2 using 16600-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPTLC3 antibody (16600-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, mouse liver tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16600-1-AP targets SPTLC3 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SPTLC3 fusion protein Ag9760 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine palmitoyltransferase, long chain base subunit 3
Synonyms C20orf38, dJ718P11, LCB 3, LCB2b, Serine palmitoyltransferase 3, SPT 3, SPTLC2L, SPTLC3
Calculated Molecular Weight 175 aa, 20 kDa
Observed Molecular Weight62 kDa
GenBank Accession NumberBC020656
Gene Symbol SPTLC3
Gene ID (NCBI) 55304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUV7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPTLC3 antibody 16600-1-APDownload protocol
IF protocol for SPTLC3 antibody 16600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Am J Cancer Res

TRIM8 inhibits breast cancer proliferation by regulating estrogen signaling.

Authors - Zelin Tian
View Article