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  • KD/KO Validated

SPTLC2 Polyclonal antibody

SPTLC2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 51012-2-AP

Print datasheet

Synonyms

KIAA0526, LCB 2, LCB2, LCB2a, Serine palmitoyltransferase 2, SPT 2, SPT2, SPTLC2



经过测试的应用

Positive WB detected inA549 cells, mouse brain tissue, HEK-293 cells, HT-29 cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51012-2-AP targets SPTLC2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IF, FC, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine palmitoyltransferase, long chain base subunit 2
Synonyms KIAA0526, LCB 2, LCB2, LCB2a, Serine palmitoyltransferase 2, SPT 2, SPT2, SPTLC2
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 55-65 kDa
GenBank Accession NumberBC005123
Gene Symbol SPTLC2
Gene ID (NCBI) 9517
RRIDAB_2195870
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15270
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPTLC2 antibody 51012-2-APDownload protocol
IHC protocol for SPTLC2 antibody 51012-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Gastroenterology

IRE1A Stimulates Hepatocyte-Derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis.

Authors - Debanjali Dasgupta
humanWB,FC

Nat Microbiol

Sphingolipid biosynthesis induces a conformational change in the murine norovirus receptor and facilitates viral infection.

Authors - Robert C Orchard
mouseWB,IF

Mol Cell Proteomics

Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.

Authors - Campbell-Valois François-Xavier FX
mouseWB

Cell Death Dis

Sphingolipid de novo biosynthesis is essential for intestine cell survival and barrier function.

Authors - Zhiqiang Li
  • KO Validated
mouseWB

J Biol Chem

Liver-specific deficiency of serine palmitoyltransferase subunit 2 decreases plasma sphingomyelin and increases apolipoprotein E levels.

Authors - Li Zhiqiang Z
mouseWB

J Biol Chem

Macrophage-specific de novo synthesis of ceramide is dispensable for inflammasome-driven inflammation and insulin-resistance in obesity.

Authors - Christina D Camell