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CoraLite®594-conjugated SPTLC1 Monoclonal antibody

SPTLC1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D11D7

Cat No : CL594-66899

Print datasheet

Synonyms

HSAN, HSAN1, HSN1, LBC1, LCB 1, LCB1, Serine palmitoyltransferase 1, SPT 1, SPT1, SPTI, SPTLC1

验证数据展示

  • IF Staining of HepG2 using CL594-66899

    IF Staining of HepG2 using CL594-66899

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 SPTLC1 antibody (CL594-66899, Clone: 2D11D7 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66899-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66899 targets SPTLC1 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen SPTLC1 fusion protein Ag7876 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serine palmitoyltransferase, long chain base subunit 1
Synonyms HSAN, HSAN1, HSN1, LBC1, LCB 1, LCB1, Serine palmitoyltransferase 1, SPT 1, SPT1, SPTI, SPTLC1
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC007085
Gene Symbol SPTLC1
Gene ID (NCBI) 10558
RRIDAB_2920053
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15269
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1).

实验方案

Product Specific Protocols
IF protocol for CL594 SPTLC1 antibody CL594-66899Download protocol
Standard Protocols
Click here to view our Standard Protocols