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验证数据展示

  • WB analysis using 84761-2-RR

    WB analysis using 84761-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84761-2-RR (SPTAN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 84761-2-RR

    WB analysis of mouse brain using 84761-2-RR

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 84761-2-RR (SPTAN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84761-2-RR

    IHC staining of mouse brain using 84761-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPTAN1 antibody included
  • IHC staining of mouse brain using 84761-2-RR

    IHC staining of mouse brain using 84761-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPTAN1 antibody included
  • IHC staining of rat brain using 84761-2-RR

    IHC staining of rat brain using 84761-2-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPTAN1 antibody included
  • Affinity and Kinetic Characterization of 84761-2-RR

    Affinity and Kinetic Characterization of 84761-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84761-2-RR against Human SPTAN1 were performed. The affinity constant is 2.59 nM.

SPTAN1 Recombinant antibody

SPTAN1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84761-2-RR

产品说明书

CloneNo. 242104B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

242104B7, Alpha II spectrin, NEAS, SPTA2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in3T3-L1 cells, mouse brain tissue, HeLa cells, Jurkat cells, MDA-MB-231 cells, rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SPTAN1 Ready-To-Use IHC Kit. SPTAN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84761-2-RR targets SPTAN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SPTAN1 fusion protein Ag36472 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
别名 242104B7, Alpha II spectrin, NEAS, SPTA2
计算分子量285kDa，2472aa
观测分子量285 kDa,150 kDa
GenBank蛋白编号NM_003127.3
基因名称 SPTAN1
Gene ID (NCBI) 6709
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPTAN1 (Nonerythroid spectrin αII), also termed α-Fodrin, is a cytoskeletal protein that belongs to the family of spectrins. Spectrins includes several structural proteins (α- and β-spectrin, α-actinin, dystrophin, and utrophin) that build and stabilize the cytoskeleton by forming a hexagonal mesh under the plasma membrane and ensuring stability and organization of organelles in the cell. SPTAN1 encodes for a 2,472 amino acid protein with a predicted molecular weight of 284 kDa and can be cleaved by calpain leading to a 150 kDa fragment (PMID: 31186638).

实验方案

Product Specific Protocols
WB protocol for SPTAN1 antibody 84761-2-RRDownload protocol
IHC protocol for SPTAN1 antibody 84761-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols