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验证数据展示

  • WB analysis of HeLa using 84761-2-RR (same clone as 84761-2-PBS)

    WB analysis of HeLa using 84761-2-RR (same clone as 84761-2-PBS)

    WB result of SPTAN1 antibody (84761-2-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTAN1 transfected HeLa cells. This data was developed using the same antibody clone with 84761-2-PBS in a different storage buffer formulation.

  • WB analysis using 84761-2-RR (same clone as 84761-2-PBS)

    WB analysis using 84761-2-RR (same clone as 84761-2-PBS)

    Various lysates were subjected to SDS-PAGE followed by western blot with 84761-2-RR (SPTAN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84761-2-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 84761-2-RR (same clone as 84761-2-PBS)

    WB analysis of mouse brain using 84761-2-RR (same clone as 84761-2-PBS)

    mouse brain tissue were subjected to SDS-PAGE followed by western blot with 84761-2-RR (SPTAN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84761-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84761-2-RR (same clone as 84761-2-PBS)

    IHC staining of mouse brain using 84761-2-RR (same clone as 84761-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84761-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPTAN1 antibody included
  • IHC staining of mouse brain using 84761-2-RR (same clone as 84761-2-PBS)

    IHC staining of mouse brain using 84761-2-RR (same clone as 84761-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84761-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPTAN1 antibody included
  • IHC staining of rat brain using 84761-2-RR (same clone as 84761-2-PBS)

    IHC staining of rat brain using 84761-2-RR (same clone as 84761-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84761-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPTAN1 antibody included
  • Affinity and Kinetic Characterization of 84761-2-RR

    Affinity and Kinetic Characterization of 84761-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84761-2-RR against Human SPTAN1 were performed. The affinity constant is 2.59 nM.

  • Featured Product
  • KD/KO验证

SPTAN1 Recombinant monoclonal antibody, PBS Only

SPTAN1 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84761-2-PBS

产品说明书

CloneNo. 242104B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

242104B7, Alpha II spectrin, NEAS, SPTA2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SPTAN1 Ready-To-Use IHC Kit. SPTAN1 primary antibody included.

产品信息

84761-2-PBS targets SPTAN1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag36472

Product name: Recombinant human SPTAN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2315-2472 aa of NM_003127.3

Sequence: RNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
别名 242104B7, Alpha II spectrin, NEAS, SPTA2
计算分子量285kDa，2472aa
观测分子量285 kDa,150 kDa
GenBank蛋白编号NM_003127.3
基因名称 SPTAN1
Gene ID (NCBI) 6709
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13813
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPTAN1 (Nonerythroid spectrin αII), also termed α-Fodrin, is a cytoskeletal protein that belongs to the family of spectrins. Spectrins includes several structural proteins (α- and β-spectrin, α-actinin, dystrophin, and utrophin) that build and stabilize the cytoskeleton by forming a hexagonal mesh under the plasma membrane and ensuring stability and organization of organelles in the cell. SPTAN1 encodes for a 2,472 amino acid protein with a predicted molecular weight of 284 kDa and can be cleaved by calpain leading to a 150 kDa fragment (PMID: 31186638).

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