验证数据展示
经过测试的应用
| Positive WB detected in | RT-4 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27370-1-AP targets SPPL2B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag25551 Product name: Recombinant human SPPL2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 40-113 aa of BC028391 Sequence: EGKDYCILYNPQWAHLPHDLSKASFLQLRNWTASLLCSAADLPARGFSNQIPLVARGNCTFYEKVRLAQGSGAR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | signal peptide peptidase-like 2B |
| 别名 | IMP 4, IMP4, Intramembrane protease 4, KIAA1532, Presenilin like protein 1 |
| 计算分子量 | 65 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC028391 |
| 基因名称 | SPPL2B |
| Gene ID (NCBI) | 56928 |
| RRID | AB_3742228 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TCT7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPPL2B (Signal Peptide Peptidase-Like 2B) is a multi-pass transmembrane aspartyl protease belonging to the GXGD family of intramembrane proteases. SPPL2B contains two conserved catalytic aspartate motifs localized within its membrane-spanning regions, similar to presenilins and γ-secretase. It localizes primarily to endosomes, lysosomes, and the plasma membrane, distinguishing it from ER-resident SPP and SPPL3. (PMID: 32052089, PMID: 19429495)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SPPL2B antibody 27370-1-AP | Download protocol |
| WB protocol for SPPL2B antibody 27370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
