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验证数据展示

  • WB analysis of human colon using 13255-1-AP (same clone as 13255-1-PBS)

    WB analysis of human colon using 13255-1-AP (same clone as 13255-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 13255-1-AP (same clone as 13255-1-PBS)

    IHC staining of human testis using 13255-1-AP (same clone as 13255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 13255-1-AP (same clone as 13255-1-PBS)

    IHC staining of human testis using 13255-1-AP (same clone as 13255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

SPPL2A Polyclonal antibody, PBS Only

SPPL2A Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13255-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13255-1-PBS targets SPPL2A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3935

Product name: Recombinant human SPPL2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 361-520 aa of BC025740

Sequence: ITKNGESIMVELAAGPFGNNEKLPVVIRVPKLIYFSVMSVCLMPVSILGFGDIIVPGLLIAYCRRFDVQTGSSYIYYVSSTVAYAIGMILTFVVLVLMKKGQPALLYLVPCTLITASVVAWRRKEMKKFWKGNSYQMMDHLDCATNEENPVISGEQIVQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal peptide peptidase-like 2A
别名
计算分子量 520 aa, 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC025740
基因名称 SPPL2A
Gene ID (NCBI) 84888
RRIDAB_2195000
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TCT8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPPL2A(Signal peptide peptidase-like 2A) is also named as IMP3(Intramembrane protease 3), PSL2(Presenilin-like protein 2) and belongs to the peptidase A22B family. It is an aspartyl intramembrane protease, has been implicated in the proteolysis of TNF-alpha, Fas Ligand and Bri2 and endogenous signal-peptide-peptidase-like2A (SPPL2a) is localised in lysosomes/late endosomes(PMID:21896273). This protein has a signal peptide with 25 amino acid and seven glycosylation sites.

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