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验证数据展示

  • WB analysis of human colon using 13255-1-AP

    WB analysis of human colon using 13255-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 13255-1-AP

    IHC staining of human testis using 13255-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 13255-1-AP

    IHC staining of human testis using 13255-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens).

SPPL2A Polyclonal antibody

SPPL2A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13255-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

IMP 3, IMP3, Intramembrane protease 3, Presenilin like protein 2, PSL2, SPP like 2A, SPPL2A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman colon tissue, Jurkat cells, mouse colon tissue
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13255-1-AP targets SPPL2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SPPL2A fusion protein Ag3935 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal peptide peptidase-like 2A
别名 IMP 3, IMP3, Intramembrane protease 3, Presenilin like protein 2, PSL2, SPP like 2A, SPPL2A
计算分子量 520 aa, 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC025740
基因名称 SPPL2A
Gene ID (NCBI) 84888
RRIDAB_2195000
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TCT8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPPL2A(Signal peptide peptidase-like 2A) is also named as IMP3(Intramembrane protease 3), PSL2(Presenilin-like protein 2) and belongs to the peptidase A22B family. It is an aspartyl intramembrane protease, has been implicated in the proteolysis of TNF-alpha, Fas Ligand and Bri2 and endogenous signal-peptide-peptidase-like2A (SPPL2a) is localised in lysosomes/late endosomes(PMID:21896273). This protein has a signal peptide with 25 amino acid and seven glycosylation sites.

实验方案

Product Specific Protocols
WB protocol for SPPL2A antibody 13255-1-APDownload protocol
IHC protocol for SPPL2A antibody 13255-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols