验证数据展示
经过测试的应用
Positive WB detected in | human placenta tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30922-1-AP targets SPP2 in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | SPP2 fusion protein Ag29299 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | secreted phosphoprotein 2, 24kDa |
别名 | SPP24, Spp 24, Secreted phosphoprotein 24, Secreted phosphoprotein 2 |
计算分子量 | 24 kDa |
观测分子量 | 24 kDa |
GenBank蛋白编号 | NM_006944 |
基因名称 | SPP2 |
Gene ID (NCBI) | 6694 |
RRID | AB_3669785 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q13103 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPP2 (Secreted phosphoprotein 24), also known as SPP24. SPP2 is a secreted phosphoprotein, which was originally isolated and purified from bovine bone density. It is widely distributed in the body, mainly produced in the liver and transported to other tissues. SPP2 is expressed in many tissues, especially in the liver. Studies have shown that the expression level of SPP2 is up-regulated in liver cirrhosis. In addition, the expression of SPP2 is significantly related to many genes, including APOA1, SLC2A2 and TTR (PMID: 38448371). SPP2 has many biological functions, including: it can inhibit the activity of cysteine protease, participate in bone formation, inhibit calcification, and regulate BMP/TGF-β signaling pathway. SPP2 may be a tumor suppressor, which inhibits the development of HCC by inhibiting BMP/TGF-β signaling pathway. The molecular weight of SPP2 is 24 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for SPP2 antibody 30922-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |