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验证数据展示

  • WB analysis using 17740-1-AP

    WB analysis using 17740-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17740-1-AP (SPOPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SPOPL Polyclonal antibody

SPOPL Polyclonal Antibody for WB, ELISA
Cat No. 17740-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

HIB homolog 2, Roadkill homolog 2, speckle type POZ protein like, Speckle-type POZ protein-like

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17740-1-AP targets SPOPL in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag11951

Product name: Recombinant human SPOPL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-392 aa of BC071613

Sequence: MSREPTPPLPGDMSTGPIAESWCYTQVKVVKFSYMWTINNFSFCREEMGEVLKSSTFSSGPSDKMKWCLRVNPKGLDDESKDYLSLYLLLVSCPKSEVRAKFKFSLLNAKREETKAMESQRAYRFVQGKDWGFKKFIRRDFLLDEANGLLPDDKLTLFCEVSVVQDSVNISGHTNTNTLKVPECRLAEDLGNLWENTRFTDCSFFVRGQEFKAHKSVLAARSPVFNAMFEHEMEESKKNRVEINDLDPEVFKEMMRFIYTGRAPNLDKMADNLLAAADKYALERLKVMCEEALCSNLSVENVADTLVLADLHSAEQLKAQAIDFINRCSVLRQLGCKDGKNWNSNQATDIMETSGWKSMIQSHPHLVAEAFRALASAQCPQFGIPRKRLKQS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 speckle-type POZ protein-like
别名 HIB homolog 2, Roadkill homolog 2, speckle type POZ protein like, Speckle-type POZ protein-like
计算分子量 392 aa, 45 kDa
观测分子量 39-45 kDa
GenBank蛋白编号BC071613
基因名称 SPOPL
Gene ID (NCBI) 339745
RRIDAB_3085537
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6IQ16
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Speckle Type POZ Protein Like (SPOPL), a member of the MATH-BTB protein family, was frst identifed in 2010 with 82.6% sequence homology with Speckle-type POZ protein (SPOP). SPOPL is an E3 ubiquitin ligase protein that ubiquitinates and degrades the target protein. SPOPL can be used as a potential prognostic biomarker for glioblastoma multiforme in clinical work and promotes the proliferation and stemness of glioma stem cells by activating the Notch signaling pathway (PMID:37523046).

实验方案

Product Specific Protocols
WB protocol for SPOPL antibody 17740-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

Polydatin exerts therapeutic effects on myelodysplastic syndrome by inhibiting the protein expression of oncogenes via hypermethylation in vitro

Authors - Qingbing Zhou
View Article