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验证数据展示

  • WB analysis using 31931-1-AP (same clone as 31931-1-PBS)

    WB analysis using 31931-1-AP (same clone as 31931-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31931-1-AP (SPINT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31931-1-PBS in a different storage buffer formulation.

  • WB analysis using 31931-1-AP (same clone as 31931-1-PBS)

    WB analysis using 31931-1-AP (same clone as 31931-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31931-1-AP (SPINT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31931-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 31931-1-AP (same clone as 31931-1-PBS)

    IF Staining of A431 using 31931-1-AP (same clone as 31931-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SPINT3 antibody (31931-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 31931-1-PBS in a different storage buffer formulation.

SPINT3 Polyclonal antibody, PBS Only

SPINT3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 31931-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

HKIB9, Kunitz-type protease inhibitor 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31931-1-PBS targets SPINT3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35289

Product name: Recombinant human SPINT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-89 aa of NM_006652.1

Sequence: RDTIKDLLPNVCAFPMEKGPCQTYMTRWFFNFETGECELFAYGGCGGNSNNFLRKEKCEKFCKFT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine peptidase inhibitor, Kunitz type, 3
别名 HKIB9, Kunitz-type protease inhibitor 3
计算分子量10 kDa
观测分子量35 kDa
GenBank蛋白编号NM_006652.1
基因名称 SPINT3
Gene ID (NCBI) 10816
RRIDAB_3742495
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP49223
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Serine peptidase inhibitor, Kunitz type 3 (SPINT3, also known as HKIB9) might be related to serine-type endopeptidase inhibitor activity, receptor antagonist activity, and transforming growth factor beta binding (PMID: 21988899). The calculated molecular weight of SPINT3 is 10 kDa, which is lower than its observed molecular weight of 30-35 kDa by SDS-PAGE detection (PMID: 21988899).

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