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验证数据展示

  • WB analysis using 68615-1-Ig

    WB analysis using 68615-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68615-1-Ig (SPIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68615-1-Ig

    WB analysis using 68615-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68615-1-Ig (SPIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

SPIN1 Monoclonal antibody

SPIN1 Monoclonal Antibody for WB, ELISA
Cat No. 68615-1-Ig

产品说明书

CloneNo. 1G8C5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, rabbit

应用

WB, ELISA

别名

OCR, Ovarian cancer related protein, SPIN, SPIN1, spindlin 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, NCCIT cells, rabbit brain tissue, SK-BR-3 cells, HeLa cells, Jurkat cells, K-562 cells, rat brain tissue, mouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68615-1-Ig targets SPIN1 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat, rabbit
免疫原

CatNo: Ag33686

Product name: Recombinant human SPIN1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-262 aa of BC013571

Sequence: MKTPFGKTPGQRSRADAGHAGVSANMMKKRTSHKKHRSSVGPSKPVSQPRRNIVGCRIQHGWKEGNGPVTQWKGTVLDQVPVNPSLYLIKYDGFDCVYGLELNKDERVSALEVLPDRVATSRISDAHLADTMIGKAVEHMFETEDGSKDEWRGMVLARAPVMNTWFYITYEKDPVLYMYQLLDDYKEGDLRIMPDSNDSPPAEREPGEVVDSLVGKQVEYAKEDGSKRTGMVIHQVEAKPSVYFIKFDDDFHIYVYDLVKTS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 spindlin 1
别名 OCR, Ovarian cancer related protein, SPIN, SPIN1, spindlin 1
计算分子量 262 aa, 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC013571
基因名称 SPIN1
Gene ID (NCBI) 10927
RRIDAB_3085309
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y657
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPIN1 (Spindlin 1) is a histone methylation reader, which recognizes trimethylated histone H3 lysine 4 through the protein-protein interaction. SPIN1 was initially found in ovarian cancer via searching ovarian cancer-related genes . It is overexpressed in ovarian cancer tissue, which exhibits a high homology to mouse Spin (Spindlin) gene. Signaling pathways responsible for SPIN1 functions include PI3K/Akt, Wnt and RET that are highly relevant to tumorigenesis.(PMID: 34492335, PMID: 31790762)

实验方案

Product Specific Protocols
WB protocol for SPIN1 antibody 68615-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols