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SPI1 Polyclonal antibody
SPI1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, RAW264.7, mouse liver tissue, rat liver tissue |
Planning an IHC experiment? We recommend our IHCeasy SPI1 Ready-To-Use IHC Kit. SPI1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55100-1-AP targets SPI1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | spleen focus forming virus (SFFV) proviral integration oncogene spi1 |
| Synonyms | 31 kDa transforming protein, OF, PU.1, SFPI1, SPI 1, SPI A, SPI1, Transcription factor PU.1 |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 31-42 kDa |
| GenBank Accession Number | NM_003120 |
| Gene Symbol | SPI1 |
| Gene ID (NCBI) | 6688 |
| ENSEMBL Gene ID | ENSG00000066336 |
| RRID | AB_10858486 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17947 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPI1 binds to the PU-box which is a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. SPI1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. It also binds RNA and may modulate pre-mRNA splicing. The antibody is specific to SPI1. SPI1 can be detected 37-42 kDa (PMID: 28362429, 31586032, 28586009).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPI1 antibody 55100-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mucosal Immunol IL-33/ST2/IL-9/IL-9R signaling disrupts ocular surface barrier in allergic inflammation. | ||
Sci Rep Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine. | ||
J Leukoc Biol miR-199a-5p inhibits monocyte/macrophage differentiation by targeting the activin A type 1B receptor gene and finally reducing C/EBPα expression.
| ||
Exp Cell Res An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1 |
