SPHK1 Recombinant antibody
SPHK1 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2K12
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, L02 cells, MCF-7 cells, NIH/3T3 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:5000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82609-1-RR targets SPHK1 in WB, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | WB, ELISA |
Tested Reactivity | Human, mouse |
Immunogen | SPHK1 fusion protein Ag1051 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | sphingosine kinase 1 |
Synonyms | SK 1, sphingosine kinase 1, SPHK, SPHK1, SPHK1 phopho, SPK, SPK 1 |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | BC008040 |
Gene Symbol | SPHK1 |
Gene ID (NCBI) | 8877 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9NYA1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).
实验方案
Product Specific Protocols | |
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WB protocol for SPHK1 antibody 82609-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |