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SPDYC Polyclonal antibody

SPDYC Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat No : 22471-1-AP

Print datasheet

Synonyms

hSpy/Ringo C, RINGO C, Ringo2, SPDYC, Speedy protein C

验证数据展示

  • IHC staining of human liver using 22471-1-AP

    IHC staining of human liver using 22471-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPDYC antibody included
  • IHC staining of human liver using 22471-1-AP

    IHC staining of human liver using 22471-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPDYC antibody included
  • IHC staining of human kidney using 22471-1-AP

    IHC staining of human kidney using 22471-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPDYC antibody included
  • IHC staining of human kidney using 22471-1-AP

    IHC staining of human kidney using 22471-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPDYC antibody included
  • IHC staining of human lung cancer using 22471-1-AP

    IHC staining of human lung cancer using 22471-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPDYC antibody included
  • IHC staining of human lung cancer using 22471-1-AP

    IHC staining of human lung cancer using 22471-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPDYC antibody included

经过测试的应用

Positive IHC detected inhuman liver tissue, human kidney tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SPDYC Ready-To-Use IHC Kit. SPDYC primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22471-1-AP targets SPDYC in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA
Tested Reactivity human
Immunogen SPDYC fusion protein Ag18126 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name speedy homolog C (Xenopus laevis)
Synonyms hSpy/Ringo C, RINGO C, Ringo2, SPDYC, Speedy protein C
Calculated Molecular Weight 293 aa, 33 kDa
Observed Molecular Weight 33-40 kDa
GenBank Accession NumberBC137244
Gene Symbol SPDYC
Gene ID (NCBI) 387778
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ5MJ68
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPDYC(Speedy protein C), also known as RINGOC and Ringo2, promotes progression through the cell cycle via binding and activation of CDK1 and CDK2. Downregulation of SPDYC inhibits cell growth, reduces the fraction of cells in G1, and increases the S and G2 populations(PMID: 18802405). SPDYC is involved in the spindle-assembly checkpoint and required for recruitment of MAD2L1, BUBR1 and BUB1 to kinetochores(Uniprot).

实验方案

Product Specific Protocols
IHC protocol for SPDYC antibody 22471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols