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验证数据展示

  • IHC staining of human liver using 22471-1-AP

    IHC staining of human liver using 22471-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPDYC antibody included
  • IHC staining of human liver using 22471-1-AP

    IHC staining of human liver using 22471-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPDYC antibody included
  • IHC staining of human kidney using 22471-1-AP

    IHC staining of human kidney using 22471-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPDYC antibody included
  • IHC staining of human kidney using 22471-1-AP

    IHC staining of human kidney using 22471-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPDYC antibody included
  • IHC staining of human lung cancer using 22471-1-AP

    IHC staining of human lung cancer using 22471-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPDYC antibody included
  • IHC staining of human lung cancer using 22471-1-AP

    IHC staining of human lung cancer using 22471-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPDYC antibody included

SPDYC Polyclonal antibody

SPDYC Polyclonal Antibody for IHC, ELISA
Cat No. 22471-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

hSpy/Ringo C, RINGO C, Ringo2, SPDYC, Speedy protein C

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman liver tissue, human kidney tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SPDYC Ready-To-Use IHC Kit. SPDYC primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22471-1-AP targets SPDYC in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18126

Product name: Recombinant human SPDYC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-293 aa of BC137244

Sequence: MLWAIPELGSPCPISISYEMSDSQDPTTSPVVTTQVELGGCSRQGGGNGFLRFRQHQEVQAFLSLLEDSFVQEFLSKDPCFQISDKYLLAMVLVYFQRAHLKLSEYTHSSLFLALYLANDMEEDLEGPKCEIFPWALGKDWCLRVGKFLHQRDKLWARMGFRAVVSRQCCEEVMAKEPFHWAWTRDRRPHHGGVQRVCPQVPVRLPRGPGLSPPHCSPCGLPQHCSSHLLKPVSSKCPSLTSECHRPPSQNYLSRVKNAWGGDFLIVLPPQMQLEPGTYSLRIFPKPPARPGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 speedy homolog C (Xenopus laevis)
别名 hSpy/Ringo C, RINGO C, Ringo2, SPDYC, Speedy protein C
计算分子量 293 aa, 33 kDa
观测分子量 33-40 kDa
GenBank蛋白编号BC137244
基因名称 SPDYC
Gene ID (NCBI) 387778
RRIDAB_3085699
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ5MJ68
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPDYC(Speedy protein C), also known as RINGOC and Ringo2, promotes progression through the cell cycle via binding and activation of CDK1 and CDK2. Downregulation of SPDYC inhibits cell growth, reduces the fraction of cells in G1, and increases the S and G2 populations(PMID: 18802405). SPDYC is involved in the spindle-assembly checkpoint and required for recruitment of MAD2L1, BUBR1 and BUB1 to kinetochores(Uniprot).

实验方案

Product Specific Protocols
IHC protocol for SPDYC antibody 22471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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