验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells, SKOV-3 cells, THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87323-1-RR targets SPC25 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag23985 Product name: Recombinant human SPC25 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC022255 Sequence: MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFAEKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTANIQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVYN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) |
| 别名 | AD024, hSpc25, Kinetochore protein Spc25, SPBC25 |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC022255 |
| 基因名称 | SPC25 |
| Gene ID (NCBI) | 57405 |
| RRID | AB_3745470 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9HBM1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Spindle Pole Body Component 25 (SPC25), a core subunit of the NDC80 kinetochore complex, is essential for accurate chromosome alignment and segregation during mitosis . By linking microtubules to kinetochores, SPC25 ensures proper attachment and tension generation, critical for the maintenance of genomic stability . SPC25 is largely found in the cytoplasm and nucleus. The NDC80 complex, which is involved in kinetochore-microtubule interactions and spindle assembly checkpoint activity, is composed of four subunits: SPC24, SPC25, NDC80, and NUF2. (PMID: 40587622 ,PMID: 40400636)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPC25 antibody 87323-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
