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验证数据展示

  • WB analysis of human testis using 16656-1-AP

    WB analysis of human testis using 16656-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 16656-1-AP

    IHC staining of human testis using 16656-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 16656-1-AP

    IHC staining of human testis using 16656-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of mouse testis using 16656-1-AP

    IF Staining of mouse testis using 16656-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPATA19 antibody (16656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse testis using 16656-1-AP

    IF Staining of mouse testis using 16656-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPATA19 antibody (16656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of mouse testis using 16656-1-AP

    WB analysis of mouse testis using 16656-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of rat testis using 16656-1-AP

    WB analysis of rat testis using 16656-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

SPATA19 Polyclonal antibody

SPATA19 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 16656-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA and More (1)

别名

Spermatogenesis-associated protein 19, mitochondrial, spermatogenesis associated 19, Spergen-1, SPERGEN1, Spergen 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman testis tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16656-1-AP targets SPATA19 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag10108

Product name: Recombinant human SPATA19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-167 aa of BC058039

Sequence: MIITTWIVYILARKGVGLPFLPITSSDIDVVESEAVSVLHHWLKKTEEEASRGIKEKLSINHPSQGVREKMSTDSPPTHGQDIHVTRDVVKHHLSKSDLLANQSQEVLEERTRIQFIRWSHTRIFQVPSEMTEDIMRDRIEQVRRSISRLTDVSAQDFSMRPSSSDC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermatogenesis associated 19
别名 Spermatogenesis-associated protein 19, mitochondrial, spermatogenesis associated 19, Spergen-1, SPERGEN1, Spergen 1
计算分子量 167 aa, 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC058039
基因名称 SPATA19
Gene ID (NCBI) 219938
RRIDAB_1851668
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z5L4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SPATA19 antibody 16656-1-APDownload protocol
IHC protocol for SPATA19 antibody 16656-1-APDownload protocol
WB protocol for SPATA19 antibody 16656-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Development

MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis

Authors -
View Article
mouseWB,IF

Development

MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.

Authors - Xiaoli Wang
View Article
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