• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SPATA19 Polyclonal antibody

SPATA19 Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16656-1-AP

Print datasheet

Synonyms

SPAS1, SPATA19, Spergen 1, spergen1, spermatogenesis associated 19

验证数据展示

  • WB analysis of human testis using 16656-1-AP

    WB analysis of human testis using 16656-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 16656-1-AP

    IHC staining of human testis using 16656-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 16656-1-AP

    IHC staining of human testis using 16656-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of mouse testis using 16656-1-AP

    IF Staining of mouse testis using 16656-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPATA19 antibody (16656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse testis using 16656-1-AP

    IF Staining of mouse testis using 16656-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPATA19 antibody (16656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of mouse testis using 16656-1-AP

    WB analysis of mouse testis using 16656-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of rat testis using 16656-1-AP

    WB analysis of rat testis using 16656-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman testis tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman testis tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16656-1-AP targets SPATA19 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen SPATA19 fusion protein Ag10108 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name spermatogenesis associated 19
Synonyms SPAS1, SPATA19, Spergen 1, spergen1, spermatogenesis associated 19
Calculated Molecular Weight 167 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC058039
Gene Symbol SPATA19
Gene ID (NCBI) 219938
RRIDAB_1851668
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z5L4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPATA19 antibody 16656-1-APDownload protocol
IHC protocol for SPATA19 antibody 16656-1-APDownload protocol
IF protocol for SPATA19 antibody 16656-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Development

MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis

Authors -
View Article
mouseWB,IF

Development

MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.

Authors - Xiaoli Wang
View Article