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验证数据展示

  • WB analysis of human heart using 13517-1-AP (same clone as 13517-1-PBS)

    WB analysis of human heart using 13517-1-AP (same clone as 13517-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 13517-1-AP (SPARCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 13517-1-AP (same clone as 13517-1-PBS)

    IHC staining of human tonsillitis using 13517-1-AP (same clone as 13517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13517-1-AP (SPARCL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 13517-1-AP (same clone as 13517-1-PBS)

    IHC staining of human tonsillitis using 13517-1-AP (same clone as 13517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13517-1-AP (SPARCL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.

  • IP experiment of Raji using 13517-1-AP (same clone as 13517-1-PBS)

    IP experiment of Raji using 13517-1-AP (same clone as 13517-1-PBS)

    IP result of anti-SPARCL1 (IP:13517-1-AP, 4ug; Detection:13517-1-AP 1:300) with Raji cells lysate 1440ug. This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.

SPARCL1 Polyclonal antibody, PBS Only

SPARCL1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13517-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13517-1-PBS targets SPARCL1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4448

Product name: Recombinant human SPARCL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 316-664 aa of BC033721

Sequence: VSEALLMEPTDDGNTTPRNHGVDDDGDDDGDDGGTDGPRHSASDDYFIPSQAFLEAERAQSIAYHLKIEEQREKVHENENIGTTEPGEHQEAKKAENSSNEEETSSEGNMRVHAVDSCMSFQCKRGHICKADQQGKPHCVCQDPVTCPPTKPLDQVCGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPTCTDFEVIQFPLRMRDWLKNILMQLYEANSEHAGYLNEKQRNKVKKIYLDEKRLLAGDHPIDLLLRDFKKNYHMYVYPVHWQFSELDQHPMDRVLTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SPARC-like 1 (hevin)
别名
计算分子量 664 aa, 75 kDa
观测分子量 130-140 kDa
GenBank蛋白编号BC033721
基因名称 SPARCL1
Gene ID (NCBI) 8404
RRIDAB_10598475
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14515
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPARC-like protein 1 (SPARCL1), also known as SC1 or Hevin, is an extracellular matrix glycoprotein that belongs to the SPARC family. SPARCL1 is widely expressed in normal and cancer tissues, and has been shown to regulate cellular adhesion and migration. SPARCL1 has been implicated in tumor initiation, formation, and progression of various cancers, including pancreatic, prostate, bladder, ovarian, breast and non-small cell lung cancers.

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